ABSTRACT
Objective To understand the main genotypes ,age distribution and infection situation of women cervical human papil‐lomavirus (HPV) infection ,and to investigate the clinical value of the HPV genotype detection combined with thinprep cytology test(TCT ) in screening the cervical lesions .Methods The detection results in 3 272 women voluntarily receiving the cervical cancer screening ,HPV genotype detection and TCT examination in the gynecology clinic of our hospital from January 2012 to December 2013 were performed the statistical analysis .Results Among 3 272 cases ,841 cases were HPV positive with the positive rate of 25 .70% and mainly concentrated in 25 - < 50 years old ,in which 714 strains were high‐risk subtype (including mixed infection) , accounting for 84 .90% ,the HPV type 16 ,52 ,58 ,18 were in the top four ,the multiple infection was in 185 cases (at least one kind of high risk type was detected out in each case of multiple infection) ;253 cases were TCT positive with the positive rate of 7 .73% , in which 167 cases were abnormal diagnosed by the pathological biopsy ,the coincidence rate reached 66 .01% ,among 167 cases of positive pathological biopsy ,166 cases were HPV infection ,7 cases of cervical cancer all were infected by high‐risk type HPV .Con‐clusion The HPV subtype detection combined with TCT can significantly increase the positive rate of cervical cancer screening , which conduces to achieve early prevention ,early discovery ,early treatment for reducing the incidence of cervical cancer .
ABSTRACT
Objective To investigate the levels of platelet-derived growth factor-BB (PDGF-BB) and its role in the pathogenesis of the patients with hepatic diseases.Methods The levels of serum PDGF-BB in 30 chronic patients (hepatitis B,26; hepatitis C,4),24 patients with post-hepatitis cirrhosis,30 patients with primary hepatocellular carcinoma,and 20 normal controls were measured by ELISA.The levels of PDGF-B mRNA in peripheral blood mononuclear cells were measured by reverse transcription polymerase chain reaction (RT-PCR).Results The serum levels of PDGF-BB and the levels of PDGF-B mRNA in peripheral blood mononuclear cells were significantly higher in the patients with hepatic diseases (F=1774.40,1037.42,P